Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Product Name | SF3B4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.1) Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008) |
| Immunogen | This SF3B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-187 amino acids from the Central region of human SF3B4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SF3B4 |
|---|---|
| Alternative Names | Splicing factor 3B subunit 4 Pre-mRNA-splicing factor SF3b 49 kDa subunit SF3b50 Spliceosome-associated protein 49 SAP 49 SF3B4 SAP49 |
| Molecular Weight(MW) | 44 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
