Sfpi1 binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Sfpi1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Sfpi1 also binds RNA and may modulate pre-mRNA splicing.1) McDevit, D.C. (2006) Mol. Immunol. 43 (10), 1541-1548.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SFPI1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SFPI1 antibody can be used for detection of SFPI1 by ELISA at 1:312500. SFPI1 antibody can be used for detection of SFPI1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Lymphnode at 5.0 ug/ml.
Positive Control
1) Cat. No. 1369 - Human Lymph node Tissue Lysate
Application Notes
SFPI1 antibody can be used for detection of SFPI1 by ELISA at 1:312500. SFPI1 antibody can be used for detection of SFPI1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SFPI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt