Application
Flow cytometric analysis of K562 cells using SFRP5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of SFRP5 Antibody in K562 cell line lysates (35ug/lane)| Product Name | SFRP5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3 |
| Product description | Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina.1) Deloukas,P., et.al., Nature 429 (6990), 375-381 (2004) |
| Immunogen | This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 amino acids from the Central region of human SFRP5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SFRP5 |
|---|---|
| Alternative Names | Secreted frizzled-related protein 5 sFRP-5 Frizzled-related protein 1b FRP-1b Secreted apoptosis-related protein 3 SARP-3 SFRP5 FRP1B SARP3 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of K562 cells using SFRP5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with SFRP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of SFRP5 Antibody in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
