SFRS8 is a human homolog of Drosophila splicing regulatory protein.This gene encodes a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Multiple alternatively spliced variants have been identified although their full-length natures have not been characterized to date.1) Olsen, J.V., (2006) Cell 127 (3), 635-648.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SFRS8.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SFRS8 antibody can be used for detection of SFRS8 by ELISA at 1:62500. SFRS8 antibody can be used for detection of SFRS8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.
Positive Control
1) 293T Cell Lysate
Application Notes
SFRS8 antibody can be used for detection of SFRS8 by ELISA at 1:62500. SFRS8 antibody can be used for detection of SFRS8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SFRS8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.