Application
SGEF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | SGEF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SGEF activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and promotes bacterial entry.1) Yamada, S., et al. Oncogene 23(35):5901-5911(2004) |
| Immunogen | This SGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-406 amino acids from the Central region of human SGEF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGEF26 |
|---|---|
| Alternative Names | Rho guanine nucleotide exchange factor 26 SH3 domain-containing guanine exchange factor ARHGEF26 SGEF |
| Molecular Weight(MW) | 97 kDa |
Application
SGEF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
