Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SGF29 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of SGF29 Antibody in MDA-MB435 cell line lysates (35ug/lane)| Product Name | SGF29 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CCDC101 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A; MIM 602276)-containing (ATAC) complex and the SPT3 (SUPT3H; MIM 602947)-TAF9 (MIM 600822)-GCN5 (KAT2A; MIM 602301)/PCAF (KAT2B; MIM 602303) acetylase (STAGA) complex. Both of these complexes contain either GCN5 or PCAF, which are paralogous acetyltransferases.1) Imielinski, M., et al. Nat. Genet. 41(12):1335-1340(2009) |
| Immunogen | This SGF29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-208 amino acids from the C-terminal region of human SGF29. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCDC101 |
|---|---|
| Alternative Names | SAGA-associated factor 29 homolog Coiled-coil domain-containing protein 101 CCDC101 SGF29 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SGF29 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of SGF29 Antibody in MDA-MB435 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
