Application
Western blot analysis in U-937 cell line lysates (35ug/lane).| Product Name | SGOL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity). Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.1) Tanno, Y., et al. Genes Dev. 24(19):2169-2179(2010) |
| Immunogen | This SGOL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids from the Central region of human SGOL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SGOL2 |
|---|---|
| Alternative Names | Shugoshin-like 2 Shugoshin-2 Sgo2 Tripin SGOL2 |
| Molecular Weight(MW) | 145 kDa |
Application
Western blot analysis in U-937 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
