Application
SGPP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.| Product Name | SGPP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM].1) Hicks, A.A., et al. PLoS Genet.(10), E1000672 (2009) : |
| Immunogen | This SGPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SGPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SGPP1 |
|---|---|
| Alternative Names | Sphingosine-1-phosphate phosphatase 1 SPPase1 Spp1 hSPP1 hSPPase1 313- Sphingosine-1-phosphatase 1 SGPP1 |
| Molecular Weight(MW) | 49 kDa |
Application
SGPP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
