Application
Immunofluorescence of SGSM1 in human brain tissue with SGSM1 antibody at 20 μg/mL.Application
Western blot analysis of SGSM1 in human cerebellum tissue lysate with SGSM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | SGSM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SGSM1 Antibody: Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation. A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. SGSM1 (RUTBC2) is a 1,148 amino acid protein that localizes to the Golgi apparatus and is mainly expressed in the CNS. SGSM1 interacts with RAP and RAB subfamily members of the small G proteins, and function as modulators of RAP and RAB-mediated neuronal signal transduction and vesicular transportation pathways.1) Bar-Sagi D and Hall A. Ras and Rho GTPases: a family reunion. Cell 2000; 103:227-38. |
| Immunogen | SGSM1 antibody was raised against a 17 amino acid synthetic peptide near the center of human SGSM1. The immunogen is located within amino acids 480 - 530 of SGSM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SGSM1 antibody can be used for detection of SGSM1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SGSM1 |
|---|---|
| Alternative Names | SGSM1 Antibody: RUTBC2 KIAA1941 RUTBC2 Small G protein signaling modulator 1 RUN and TBC1 domain-containing protein 2 |
| Molecular Weight(MW) | 126 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least four isoforms of SGSM1 are known to exist; SGSM1 antibody will detect all four isoforms. |
| Entrez Gene | 129049 |
|---|---|
| Protein Accession | NP_001035037 |
Application
Immunofluorescence of SGSM1 in human brain tissue with SGSM1 antibody at 20 μg/mL.
Application
Western blot analysis of SGSM1 in human cerebellum tissue lysate with SGSM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1362 - Human Cerebellum Tissue Lysate |
|---|---|
| Application Notes | SGSM1 antibody can be used for detection of SGSM1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SGSM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SGSM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
