Application
Immunofluorescence of SGSM2 in human brain tissue with SGSM2 antibody at 20 μg/mL.Application
Western blot analysis of SGSM2 in human cerebellum tissue lysate with SGSM2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide| Product Name | SGSM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SGSM2 Antibody: Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation. A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. SGSM2 is a Rab9A effector that activates GTP hydrolysis by Rab32 and Rab33B proteins.1) Bar-Sagi D and Hall A. Ras and Rho GTPases: a family reunion. Cell 2000; 103:227-38. |
| Immunogen | SGSM2 antibody was raised against an 18 amino acid synthetic peptide near the center of human SGSM2. The immunogen is located within amino acids 650 - 700 of SGSM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SGSM2 antibody can be used for detection of SGSM2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SGSM2 |
|---|---|
| Alternative Names | SGSM2 Antibody: RUTBC1 KIAA0397 RUTBC1 Small G protein signaling modulator 2 RUN and TBC1 domain-containing protein 1 |
| Molecular Weight(MW) | 116 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | Multiple isoforms of SGSM2 are known to exist. SGSM2 antibody is predicted to not cross react with other SGSM family proteins. |
Application
Immunofluorescence of SGSM2 in human brain tissue with SGSM2 antibody at 20 μg/mL.
Application
Western blot analysis of SGSM2 in human cerebellum tissue lysate with SGSM2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1362 - Human Cerebellum Tissue Lysate |
|---|---|
| Application Notes | SGSM2 antibody can be used for detection of SGSM2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SGSM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SGSM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
