SGSM3 Antibody: Small G proteins such as RAP and RAB proteins are the key molecules in intracellular signal transduction and vesicle transportation. A novel protein family small G protein signaling modulator (SGSM) consisting of three members SGSM1-3 bind to RAP and RAB family proteins. All three SGSM proteins possess both a RUN domain and a TBC domain. Little is known of the exact function of SGSM3.1) Bar-Sagi D and Hall A. Ras and Rho GTPases: a family reunion. Cell 2000; 103:227-38.
Immunogen
SGSM3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human SGSM3. The immunogen is located within amino acids 30 - 80 of SGSM3.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIFWB
SGSM3 antibody can be used for detection of SGSM3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Immunofluorescence of SGSM3 in 293 cells with SGSM3 antibody at 20 μg/mL.
Application
Western blot analysis of SGSM3 in 293 cell lysate with SGSM3 antibody at (A) 1 and (B) 2 μg/mL.
Positive Control
1) Cat. No. 1210 - HEK293 Cell Lysate
Application Notes
SGSM3 antibody can be used for detection of SGSM3 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Additional Information
Form
Liquid
Storage Instructions
SGSM3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
SGSM3 Antibody is supplied in PBS containing 0.02% sodium azide.