Application
Immunohistochemistry of SH3BP4 in human lung tissue with SH3BP4 antibody at 5 μg/mL.Application
Western blot analysis of SH3BP4 in rat lung tissue lysate with SH3BP4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SH3BP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SH3BP4 Antibody: SH3BP4 was initially isolated as a novel cDNA using differential display analysis using cultured human corneal fibroblasts. The protein contains three Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. SH3BP4 has been shown to interact with specific endocytic proteins such as clathrin, dynamin, and the transferrin receptor (TfR) and localizes to TfR-containing coated pits and vesicles. It is thought to be involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of TfR. At least two isoforms of SH3BP4 are known to exist.1) Dunlevy JR, Berryhill BL, Vergnes JP, et al. Cloning, chromosomal localization, and characterization of cDNA from a novel gene, SH3BP4, expressed by human corneal fibroblasts. Genomics1999; 62:519-24. |
| Immunogen | SH3BP4 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human SH3BP4. The immunogen is located within amino acids 860 - 910 of SH3BP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| SH3BP4 antibody can be used for detection of SH3BP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SH3BP4 |
|---|---|
| Alternative Names | SH3BP4 Antibody: TTP BOG25 EHB10 TTP SH3 domain-binding protein 4 EH-binding protein 10 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Immunohistochemistry of SH3BP4 in human lung tissue with SH3BP4 antibody at 5 μg/mL.
Application
Western blot analysis of SH3BP4 in rat lung tissue lysate with SH3BP4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | SH3BP4 antibody can be used for detection of SH3BP4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SH3BP4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SH3BP4 Antibody is supplied in PBS containing 0.02% sodium azide. |
