Application
Immunohistochemistry of SH3RF2 in human brain tissue with SH3RF2 antibody at 10 μg/mL.Application
Immunofluorescence of SH3RF2 in human brain tissue with SH3RF2 antibody at 20 μg/mL.Application
Western blot analysis of SH3RF2 in 3T3 cell lysate with SH3RF2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SH3RF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SH3RF2 Antibody: Although the SH3 domain containing ring finger 2 protein (SH3RF2) has been identified as a marker of the nucleus accumbens in the human brain, little is known about this protein. Recent yeast two-hybrid experiments have shown that SH3RF2 associates with protein phosphatase 1 (PP1) and is expressed primarily in heart and testis tissue. Recombinant SH3RF2 enhanced PP1 enzymatic activity and antagonized the ability of phopho-inhibitor-1 or -2 to inhibit PP1, suggesting that SH3RF2 may play a role working with PP1 in cardiac functions.1) Ernst C, Sequeira A, Klempan, et al. Confirmation of region-specific patterns of gene expression in the human brain. Neurogenetics2007; 8:219-24. |
| Immunogen | SH3RF2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SH3RF2. The immunogen is located within the last 50 amino acids of SH3RF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SH3RF2 antibody can be used for detection of SH3RF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SH3RF2 |
|---|---|
| Alternative Names | SH3RF2 Antibody: HEPP1 POSHER RNF158 PPP1R39 Putative E3 ubiquitin-protein ligase SH3RF2 Heart protein phosphatase 1-binding protein HEPP1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of SH3RF2 in human brain tissue with SH3RF2 antibody at 10 μg/mL.
Application
Immunofluorescence of SH3RF2 in human brain tissue with SH3RF2 antibody at 20 μg/mL.
Application
Western blot analysis of SH3RF2 in 3T3 cell lysate with SH3RF2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | SH3RF2 antibody can be used for detection of SH3RF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SH3RF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SH3RF2 Antibody is supplied in PBS containing 0.02% sodium azide. |
