Application
Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.Application
Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.| Product Name | SHANK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHANK3 Antibody: SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.1) Sheng M and Kim E. The Shank family of scaffold proteins. J. Cell Sci. 2000; 113:1851-6. |
| Immunogen | SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3. The immunogen is located within amino acids 890 - 940 of SHANK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHANK3 |
|---|---|
| Alternative Names | SHANK3 Antibody: PSAP2 SCZD15 PROSAP2 SPANK-2 DEL22q13.3 KIAA1650 PSAP2 SH3 and multiple ankyrin repeat domains protein 3 Proline-rich synapse-associated protein 2 Shank3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three alternatively spliced transcript isoforms of SHANK3 are known to exist. |
| Entrez Gene | 85358 |
|---|---|
| Protein Accession | NP_001073889 |
Application
Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.
Application
Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHANK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide. |
