Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Phospho-Tyr427). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 (Phospho-Tyr427).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using Shc1 (Phospho-Tyr427).| Product Name | Shc1 (phospho Tyr427) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span1) Trampont P, et al. (2006) Mol Cell Biol; 26 (23): 9035-9044. Patrussi L, et al. (2005) Oncogene; 24 (13): 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11): 1435-1444 |
| Immunogen | Shc1 (Phospho-Tyr427) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y (p) -V-N derived from Human Shc1. |
| Modification | p-Tyr427 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHC1 |
|---|---|
| Alternative Names | SHC SHCA PTP2C SHPTP2 SH2 domain protein C1 SHC SHC-transforming protein 1 Src homology 2 domain-containing-transforming protein C1 |
| Molecular Weight(MW) | 46, 52 kDa |
| Tissue Specificity | This antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427. |
| Entrez Gene | 6464 |
|---|---|
| Protein Accession | NP_001123512.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Phospho-Tyr427). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 (Phospho-Tyr427).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using Shc1 (Phospho-Tyr427).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
