Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Product Name | SHC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHC1 is a signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.1) George, R., (2008) J. Mol. Biol. 377 (3), 740-747. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SHC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SHC1 antibody can be used for detection of SHC1 by ELISA at 1:312500. SHC1 antibody can be used for detection of SHC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHC1 |
|---|---|
| Alternative Names | SHC1 FLJ26504 SHC SHCA p52SHC p66 p66SHC |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate |
|---|---|
| Application Notes | SHC1 antibody can be used for detection of SHC1 by ELISA at 1:312500. SHC1 antibody can be used for detection of SHC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SHC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
