Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SHCBP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | SHCBP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 1) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : |
| Immunogen | This SHCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-528 amino acids from the C-terminal region of human SHCBP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHCBP1 |
|---|---|
| Alternative Names | SHC SH2 domain-binding protein 1 SHCBP1 |
| Molecular Weight(MW) | 76 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SHCBP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
