Application
Immunohistochemistry of SHISA3 in human small intestine tissue with SHISA3 antibody at 2.5 μg/ml.Application
Immunofluorescence of SHISA3 in human small intestine tissue with SHISA3 antibody at 20 μg/ml.Application
Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SHISA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHISA3 Antibody: SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.1) Furushima K, Yamamoto A, Nagano T, et al. Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev. Biol. 2007; 306:480-92. |
| Immunogen | SHISA3 antibody was raised against a 15 amino acid peptide near the center of human SHISA3 . The immunogen is located within amino acids 120 - 170 of SHISA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SHISA3 Antibody can be used for detection of SHISA3 by Western blot at 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHISA3 |
|---|---|
| Alternative Names | SHISA3 Antibody: hShisa3 Protein shisa-3 homolog |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 28 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | SHISA3 antibody is predicted to not cross-react with other SHISA protein family members. |
| Entrez Gene | 152573 |
|---|---|
| Protein Accession | NP_001073974 |
Application
Immunohistochemistry of SHISA3 in human small intestine tissue with SHISA3 antibody at 2.5 μg/ml.
Application
Immunofluorescence of SHISA3 in human small intestine tissue with SHISA3 antibody at 20 μg/ml.
Application
Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | SHISA3 Antibody can be used for detection of SHISA3 by Western blot at 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHISA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SHISA3 Antibody is supplied in PBS containing 0.02% sodium azide. |
