Application
Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.Application
Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.Application
Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SHISA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHISA4 Antibody: SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.1) Furushima K, Yamamoto A, Nagano T, et al. Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev. Biol. 2007; 306:480-92. |
| Immunogen | SHISA4 antibody was raised against a 15 amino acid peptide near the center of human SHISA4 . The immunogen is located within amino acids 100 - 150 of SHISA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHISA4 |
|---|---|
| Alternative Names | SHISA4 Antibody: TMEM58 C1orf40 TMEM58 UNQ583/PRO1153 Protein shisa-4 Transmembrane protein 58 |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 24 kDa |
| Tissue Specificity | SHISA4 antibody is predicted to not cross-react with other SHISA protein family members. |
Application
Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.
Application
Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.
Application
Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHISA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SHISA4 Antibody is supplied in PBS containing 0.02% sodium azide. |
