Application
Western blot analysis of SHOC2 in Jurkat cell lysate with SHOC2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of SHOC2 in human spleen tissue with SHOC2 antibody at 5 μg/mL.| Product Name | SHOC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHOC2 Antibody: SHOC2 protein participates in protein binding / transferase activity in the fibroblast growth factor receptor signaling pathway and Ras protein signal transduction. It is a widely expressed protein composed almost entirely of leucine-rich repeats (LRR), with a lysine-rich sequence at the amino terminus and cytoplasmically localized. SHOC2 acts as a positive modulator of the RAS-MAPK signaling cascade, which is elicited by EGL-15 and LET-23 and mediated by LET-60. SHOC2 together with protein phosphatase 1c (PP1c) forms a highly specific M-Ras effector complex and is essential for activation of the MAPK pathway by growth factors. Furthermore, in tumor cells with Ras gene mutations, inhibition of SHOC2 expression inhibits MAPK, but not PI3K activity. The SHOC2-PP1c holoenzyme provides an attractive therapeutic target for inhibition of the MAPK pathway in cancer. Recent studies show that aberrantly acquired N-myristoylation of SHOC2 causes human disease Noonan-like syndrome with loose anagen hair.1) Selfors LM, Schutzman JL, Borland CZ, et al. Soc-2 encodes a leucine-rich repeat protein implicated in fibroblast growth factor receptor signaling. Proc. Natl. Acad. Sci. USA1998; 95:6903-8. |
| Immunogen | SHOC2 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human SHOC2. The immunogen is located within amino acids 30 - 80 of SHOC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| SHOC2 antibody can be used for detection of Shoc2 by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHOC2 |
|---|---|
| Alternative Names | SHOC2 Antibody: SOC2 SUR8 SIAA0862 KIAA0862 Leucine-rich repeat protein SHOC-2 Protein soc-2 homolog |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Western blot analysis of SHOC2 in Jurkat cell lysate with SHOC2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of SHOC2 in human spleen tissue with SHOC2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SHOC2 antibody can be used for detection of Shoc2 by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHOC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHOC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
