Application
Immunofluorescence of SHQ1 in human heart tissue with SHQ1 antibody at 20 μg/mL.Application
Western blot analysis of SHQ1 in human heart tissue lysate with SHQ1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SHQ1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHQ1 Antibody: SHQ1 is the human homolog of yeast Shq1p, a protein that acts as an accessory factor in the assembly of H/ACA ribonucleoproteins. SHQ1 has been shown to bind the major H/ACA core protein and the pseudouridine synthase NAP57, but precedes the assembly role of NAF1 and of the other H/ACA core proteins. SHQ1 assists in the assembly of H/ACA-box ribonucleoproteins that function in the processing of ribosomal RNAs, modification of spliceosomal small nuclear RNAs, and stabilization of telomerase.1) Ito T, Chiba T, Ozawa R, et al. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc. Natl. Acad. Sci. USA 2001; 98:4569-74. |
| Immunogen | SHQ1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human SHQ1. The immunogen is located within amino acids 80 - 130 of SHQ1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SHQ1 antibody can be used for detection of SHQ1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHQ1 |
|---|---|
| Alternative Names | SHQ1 Antibody: Shq1p Protein SHQ1 homolog |
| Molecular Weight(MW) | 63 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | Multiple isoforms are known to exist. |
Application
Immunofluorescence of SHQ1 in human heart tissue with SHQ1 antibody at 20 μg/mL.
Application
Western blot analysis of SHQ1 in human heart tissue lysate with SHQ1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | SHQ1 antibody can be used for detection of SHQ1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHQ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHQ1 Antibody is supplied in PBS containing 0.02% sodium azide. |
