Application
Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SHROOM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHROOM1 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.1) Lee C, Le MP, and Wallingford JB. The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets. Dev. Dyn. 2009; 238:1480-91. |
| Immunogen | Rabbit polyclonal SHROOM1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human SHROOM1. The immunogen is located within the last 50 amino acids of SHROOM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHROOM1 |
|---|---|
| Alternative Names | SHROOM1 Antibody: APXL2 APXL2 KIAA1960 Protein Shroom1 Apical protein 2 |
| Molecular Weight(MW) | 94 kDa |
| Tissue Specificity | SHROOM1 antibody is human, mouse and rat reactive. At least three isoforms of SHROOM1 are known to exist; this antibody will detect only the two largest isoforms. SHROOM1 antibody is predicted to not cross-react with other SHROOM family members. |
| Entrez Gene | 134549 |
|---|---|
| Protein Accession | NP_001166171 |
Application
Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHROOM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHROOM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
