Application
Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 μg/mL.| Product Name | SHROOM3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SHROOM3 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM3 is an actin-binding protein is a regulator of the microtubule cytoskeleton during epithelial morphogenesis and is sufficient and necessary to induce a redistribution of the microtubule regulator gamma-tubulin. It is necessary for both apiscobasal cell elongation and apical constriction in the neural epithelium. SHROOM3 has also been shown to be essential for cell shape changes and morphogenesis in the developing vertebrate gut, with its transcription relying on the Pitx1 transcription factor.1) Lee C, Le MP, and Wallingford JB. The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets. Dev. Dyn. 2009; 238:1480-91 |
| Immunogen | Rabbit polyclonal SHROOM3 antibody was raised against an 18 amino acid peptide near the center of human SHROOM3. The immunogen is located within amino acids 850 - 900 of SHROOM3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SHROOM3 antibody can be used for detection of SHROOM3 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHROOM3 |
|---|---|
| Alternative Names | SHROOM3 Antibody: SHRM APXL3 ShrmL MSTP013 KIAA1481 SHRML Protein Shroom3 Shroom-related protein hShrmL |
| Molecular Weight(MW) | 220 kDa |
| Tissue Specificity | SHROOM3 antibody is human specific. At least four isoforms of SHROOM3 are known to exist; this antibody will detect all four isoforms. SHROOM3 antibody is predicted to not cross-react with other SHROOM family members. |
Application
Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | SHROOM3 antibody can be used for detection of SHROOM3 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SHROOM3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SHROOM3 Antibody is supplied in PBS containing 0.02% sodium azide. |
