Application
Immunohistochemistry of Siglec11 in human liver tissue with Siglec11 antibody at 5 μg/mL.Application
Immunofluorescence of SIGLEC11 in human liver tissue with SIGLEC11 antibody at 20 μg/mL.Application
Western blot analysis of Siglec11 in HepG2 cell lysate with Siglec11 antibody at 1 μg/mL.| Product Name | Siglec11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Siglec11 Antibody: Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10.1) Crocker PR and Varki A. Siglecs, sialic acids, and innate immunity. Trends Immunol.2001; 22:337-42. |
| Immunogen | Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11. The immunogen is located within the last 50 amino acids of Siglec11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μg/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIGLEC11 |
|---|---|
| Alternative Names | Siglec11 Antibody: Sialic acid-binding Ig-like lectin 11 Sialic acid-binding lectin 11 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of Siglec11 in human liver tissue with Siglec11 antibody at 5 μg/mL.
Application
Immunofluorescence of SIGLEC11 in human liver tissue with SIGLEC11 antibody at 20 μg/mL.
Application
Western blot analysis of Siglec11 in HepG2 cell lysate with Siglec11 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μg/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Siglec11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Siglec11 Antibody is supplied in PBS containing 0.02% sodium azide. |
