Application
Western blot analysis of SIGLEC15 in human kidney tissue lysate with SIGLEC15 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of SIGLEC15 in human kidney tissue with SIGLEC15 antibody at 2.5 μg/mL.| Product Name | SIGLEC15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.1) Crocker PR and Redelinghuys P. Siglecs as positive and negative regulators of the immune system. Biochem. Soc. Trans. 2008; 36:1467-71. |
| Immunogen | SIGLEC15 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15. The immunogen is located within amino acids 50 - 100 of SIGLEC15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIGLEC15 |
|---|---|
| Alternative Names | SIGLEC15 Antibody: CD33L3 HsT1361 SIGLEC-15 CD33L3 Sialic acid-binding Ig-like lectin 15 CD33 antigen-like 3 Siglec-15 |
| Molecular Weight(MW) | Predicted: 36 kDa Observed: 42 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members. |
Application
Western blot analysis of SIGLEC15 in human kidney tissue lysate with SIGLEC15 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of SIGLEC15 in human kidney tissue with SIGLEC15 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIGLEC15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide. |
