Application
This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.| Product Name | sIL-2Ra Antibody [SB-647] |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | sIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents. Western Blot: To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL2RA |
|---|---|
| Alternative Names | Interleukin-2 receptor subunit alpha TAC antigen IL-2 receptor subunit alpha CD25 IL2R TCGFR IDDM10 |
Application
This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents. Western Blot: To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | sIL-2Ra lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
