Sin3B is one of the Sin3 co-repressors act as a protein scaffold to recruit transcription factors via its four highly homologous paired amphipathic helix (PAH) domains.1) Nomura, M., (2005) J. Mol. Biol. 354 (4), 903-915.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Sin3b.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
Sin3b antibody can be used for detection of Sin3b by ELISA at 1:1562500. Sin3b antibody can be used for detection of Sin3b by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1409 - Mouse Thymus Tissue Lysate
Application Notes
Sin3b antibody can be used for detection of Sin3b by ELISA at 1:1562500. Sin3b antibody can be used for detection of Sin3b by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store Sin3b antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.