Application
Immunofluorescence of SIPA1 in human brain cells with SIPA1 antibody at 20 μg/mL.Application
Western blot analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 μg/mL.| Product Name | SIPA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIPA1 Antibody: Signal-induced proliferation 1 (SIPA1) is a member of the SIPA1 family of RapGAPs, a family of mitogen-induced GTPase activating proteins. SIPA1 exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. When abnormally or prematurely expressed, SIPA1 may also hamper mitogen-induced cell cycle progression and may increase metastases in human cancers.1) Hattori M, Tsukamoto N, Nur-e-Kamal MS, et al. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol. Cell. Biol. 1995; 15:551-60. |
| Immunogen | SIPA1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human SIPA1. The immunogen is located within amino acids 40 - 90 of SIPA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| SIPA1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIPA1 |
|---|---|
| Alternative Names | SIPA1 Antibody: SPA1 SPA1 Signal-induced proliferation-associated protein 1 GTPase-activating protein Spa-1 Sipa-1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (95%) |
| Tissue Specificity | At least three isoforms of SIPA1 are known to exist; this antibody will recognize all three isoforms. This antibody is specific for SIPA1 and will not recognize the other SIPA1 family of proteins. |
Application
Immunofluorescence of SIPA1 in human brain cells with SIPA1 antibody at 20 μg/mL.
Application
Western blot analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | SIPA1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIPA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIPA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
