Application
Immunohistochemistry of SIPA1L1 in human brain tissue with SIPA1L1 antibody at 5 μg/mL.Application
Immunofluorescence of SIPA1L1 in human brain tissue with SIPA1L1 antibody at 20 μg/mL.Application
Western blot analysis of SIPA1L1 in rat brain tissue lysate with SIPA1L1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SIPA1L1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIPA1L1 Antibody: Signal-induced proliferation associated-like protein 1 (SIPA1L1) is a member of the SIPA1 family of RapGAPs. SIPA1L1 was initially identified as a binding partner and degradation target of the E6 oncoprotein of high-risk papillomaviruses. Recently, it was discovered that Casein kinase I epsilon (CKIe), a Wnt-regulated kinase that regulates Wnt/b-catenin signaling, also can bind to the carboxy-terminus of SIPA1L1. CKIe phosphorylates SIPA1L1, thereby reducing its stability and alleviating its inhibition of Rap1, a protein required for Wnt8/CKIe-mediated gastrulation during embryogenesis, suggesting SIPA1L1 plays important roles in embryo development as well as control of cell proliferation.1) Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23. |
| Immunogen | SIPA1L1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SIPA1L1. The immunogen is located within amino acids 40 - 90 of SIPA1L1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SIPA1L1 antibody can be used for detection of SIPA1L1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIPA1L1 |
|---|---|
| Alternative Names | SIPA1L1 Antibody: E6TP1 E6TP1 KIAA0440 Signal-induced proliferation-associated 1-like protein 1 High-risk human papilloma viruses E6 oncoproteins targeted protein 1 SIPA1-like protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of SIPA1L1 in human brain tissue with SIPA1L1 antibody at 5 μg/mL.
Application
Immunofluorescence of SIPA1L1 in human brain tissue with SIPA1L1 antibody at 20 μg/mL.
Application
Western blot analysis of SIPA1L1 in rat brain tissue lysate with SIPA1L1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SIPA1L1 antibody can be used for detection of SIPA1L1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIPA1L1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIPA1L1 Antibody is supplied in PBS containing 0.02% sodium azide. |
