Application
Immunohistochemistry of SIPA1L3 in human brain tissue with SIPA1L3 antibody at 5 μg/mL.Application
Immunofluorescence of SIPA1L3 in Human Brain cells with SIPA1L3 antibody at 20 μg/mL.Application
Western blot analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 μg/mL.| Product Name | SIPA1L3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIPA1L3 Antibody: Signal-induced proliferation associated-like protein 2 (SIPA1L3) is a member of the SIPA1 family of RapGAPs. SIPA1L3 was cloned as a novel molecular component of tight junctions and adherens junctions, suggesting that may function to balance Rap1 by counteracting RapGEFs. Recent studies of SIPA indicate that its deregulation can cause myeloproliferative stem cell disorders in mice and increased metastases in human cancers. Other studies suggest SIPA1L1 may play important roles in embryo development and control of cell proliferation. Based on the amount of homology between SIPA family members, it is possible that SIPA1L3 plays other roles development and cell proliferation.1) Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23. |
| Immunogen | SIPA1L3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SIPA1L3. The immunogen is located within amino acids 80 - 130 of SIPA1L3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SIPA1L3 antibody can be used for detection of SIPA1L3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIPA1L3 |
|---|---|
| Alternative Names | SIPA1L3 Antibody: SPAL3 KIAA0545 SPAL3 Signal-induced proliferation-associated 1-like protein 3 SPA-1-like protein 3 SIPA1-like protein 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of SIPA1L3 in human brain tissue with SIPA1L3 antibody at 5 μg/mL.
Application
Immunofluorescence of SIPA1L3 in Human Brain cells with SIPA1L3 antibody at 20 μg/mL.
Application
Western blot analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | SIPA1L3 antibody can be used for detection of SIPA1L3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIPA1L3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIPA1L3 Antibody is supplied in PBS containing 0.02% sodium azide. |
