Application
Western blot analysis of extracts from JK tissue using SirT1 (Phospho-Ser47) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | SirT1 (phospho Ser47) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively.1) Gao Z, et al. (2011) J Biol Chem 286, 22227-34. Zschoernig B, Mahlknecht U (2009)Biochem Biophys Res Commun 381, 372-7. Nasrin N, et al. (2009)PLoS One 4, e8414. |
| Immunogen | SirT1 (phospho Ser47) antibody was raised against a peptide sequence around phosphorylation site of serine 47 (E-R-S (p)-P-G) derived from Human SirT1. |
| Modification | p-Ser47 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT1 |
|---|---|
| Alternative Names | hSIR2 hSIRT1 NAD-dependent deacetylase sirtuin 1 SIR1 SIR2L1 |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | The antibody detects endogenous level of SirT1 only when phosphorylated at serine 47. |
| Entrez Gene | 23411 |
|---|---|
| Protein Accession | NP_001135970.1 |
Application
Western blot analysis of extracts from JK tissue using SirT1 (Phospho-Ser47) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
