Application
Antibody used in WB on Human Thymus at 0.5 ug/ml.| Product Name | SIRT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.1) Solomon, J.M., et al., (2006) Mol. Cell. Biol. 26 (1), 28-38. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIRT1 antibody can be used for detection of SIRT1 by ELISA at 1:62500. SIRT1 antibody can be used for detection of SIRT1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIRT1 |
|---|---|
| Alternative Names | SIRT1 SIR2L1 |
| Molecular Weight(MW) | 82 kDa |
Application
Antibody used in WB on Human Thymus at 0.5 ug/ml.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | SIRT1 antibody can be used for detection of SIRT1 by ELISA at 1:62500. SIRT1 antibody can be used for detection of SIRT1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
