Application
Immunohistochemical staining of Human Brain tissue using SIRT2 antibody at 2.5 μg/mL.Application
Immunofluorescence of SIRT2 in Human Brain tissue with SIRT2 antibody at 5 μg/mL.Application
Western blot analysis of SIRT2 in Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SIRT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIRT2 Antibody: The founding member of the sirtuin protein family was the silent information regulator 2 protein (Sir2p) of Saccharomyces cervisiae, an NAD+-dependent histone deacetylase (HDAC) that regulates chromatin silencing. The SIR2 family of genes are highly conserved from prokaryotes to eukaryotes. Mammals have seven homologs of Sir2p, SIRT1-7, which are involved in diverse processes ranging from transcriptional regulation, cell cycle progression and DNA-damage repair to aging. SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules and can deacetylate alpha-tubulin and regulate progression through the cell cycle. Most Sirtuins are highly expressed in brain and testis, while Sirt2 expression is higher in fetal relative to adult brain. Recent studies on SIRT2 support the therapeutic utility of inhibitors for the treatment of neurodegenerative diseases such as Parkinson's disease.1) Yamamoto H, Schoonjans K, and Auwerx J. Sirtuin functions in health and disease. Mol. Endocrinol.2007; 21:1745-55. |
| Immunogen | SIRT2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human SIRT2. The immunogen is located within the last 50 amino acids of SIRT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SIRT2 antibody can be used for detection of SIRT2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT2 |
|---|---|
| Alternative Names | SIRT2 Antibody: SIR2 SIR2L SIR2L2 NAD-dependent protein deacetylase sirtuin-2 Regulatory protein SIR2 homolog 2 |
| Molecular Weight(MW) | Predicted: 43 kDa Observed: 52 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemical staining of Human Brain tissue using SIRT2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SIRT2 in Human Brain tissue with SIRT2 antibody at 5 μg/mL.
Application
Western blot analysis of SIRT2 in Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | SIRT2 antibody can be used for detection of SIRT2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIRT2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIRT2 Antibody is supplied in PBS containing 0.02% sodium azide. |
