Application
Immunofluorescence of sirt2 in human brain tissue with sirt2 antibody at 20 μg/mL.Application
Immunohistochemical staining of human brain tissue using Sirt2 antibody at 2.5 μg/mL.Application
Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 μg/mL.| Product Name | SIRT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIRT2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.1) Yamamoto H, Schoonjans K, and Auwerx J. Sirtuin functions in health and disease. Mol. Endocrinol.2007; 21:1745-55. |
| Immunogen | SIRT2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2. The immunogen is located within the first 50 amino acids of SIRT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SIRT2 antibody can be used for detection of SIRT2 by Western blot at 2.5 - 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT2 |
|---|---|
| Alternative Names | SIRT2 Antibody: SIR2 SIR2L SIR2L2 NAD-dependent protein deacetylase sirtuin-2 Regulatory protein SIR2 homolog 2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of sirt2 in human brain tissue with sirt2 antibody at 20 μg/mL.
Application
Immunohistochemical staining of human brain tissue using Sirt2 antibody at 2.5 μg/mL.
Application
Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | SIRT2 antibody can be used for detection of SIRT2 by Western blot at 2.5 - 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SIRT2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SIRT2 Antibody is supplied in PBS containing 0.02% sodium azide. |
