Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Product Name | SIRT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIRT2 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by SIRT2 is included in class I of the sirtuin family.1) Borra, M.T., et al., (2004) Biochemistry 43 (30), 9877-9887. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIRT2 antibody can be used for detection of SIRT2 by ELISA at 1:312500. SIRT2 antibody can be used for detection of SIRT2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT2 |
|---|---|
| Alternative Names | SIRT2 SIR2 SIR2L SIR2L2 |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
|---|---|
| Application Notes | SIRT2 antibody can be used for detection of SIRT2 by ELISA at 1:312500. SIRT2 antibody can be used for detection of SIRT2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
