Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.| Product Name | SIRT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIRT4 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT4 is included in class IV of the sirtuin family. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.1) Ahuja, N., (2007) J. Biol. Chem. 282 (46), 33583-33592 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIRT4 antibody can be used for detection of SIRT4 by ELISA at 1:62500. SIRT4 antibody can be used for detection of SIRT4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT4 |
|---|---|
| Alternative Names | SIRT4 MGC130046 MGC130047 MGC57437 SIR2L4 sirtuin 4 |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate |
|---|---|
| Application Notes | SIRT4 antibody can be used for detection of SIRT4 by ELISA at 1:62500. SIRT4 antibody can be used for detection of SIRT4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
