Application
Antibody used in WB on Human heart at 0.2-1 ug/ml.Application
Antibody used in IHC.| Product Name | SIRT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SIRT5 is included in class III of the sirtuin family which ischaracterized by a sirtuin core domain. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.1) Chowdari, K.V., (2007) Genes Brain Behav. 6 (3), 229-239. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIRT5 antibody can be used for detection of SIRT5 by ELISA at 1:62500. SIRT5 antibody can be used for detection of SIRT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT5 |
|---|---|
| Alternative Names | SIRT5 SIR2L5 |
| Molecular Weight(MW) | 34 kDa |
Application
Antibody used in WB on Human heart at 0.2-1 ug/ml.
Application
Antibody used in IHC.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | SIRT5 antibody can be used for detection of SIRT5 by ELISA at 1:62500. SIRT5 antibody can be used for detection of SIRT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
