WB
Western blot analysis of SIRT5 on different lysates with Rabbit anti-SIRT5 antibody at 1/2,000 dilution. Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SIRT5 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SIRT5 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIRT5 |
|---|---|
| Gene Synonyms | SIR2L5 |
| Gene Full Name | sirtuin 5 |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| Alternative Names | NAD dependent deacetylase sirtuin 5 antibody NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody NAD dependent protein deacylase sirtuin 5 mitochondrial antibody NAD-dependent protein deacylase sirtuin-5 mitochondrial antibody Regulatory protein SIR2 homolog 5 antibody Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody Sir2 like 5 antibody SIR2-like protein 5 antibody SIR2L5 antibody SIR5_HUMAN antibody NAD dependent deacetylase sirtuin 5 antibody NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody NAD dependent protein deacylase sirtuin 5 mitochondrial antibody NAD-dependent protein deacylase sirtuin-5 mitochondrial antibody Regulatory protein SIR2 homolog 5 antibody Silent mating type information regulation 2 S.cerevisiae homolog 5 antibody Sir2 like 5 antibody SIR2-like protein 5 antibody SIR2L5 antibody SIR5_HUMAN antibody Sirt5 antibody Sirtuin 5 antibody Sirtuin type 5 antibody |
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm,Mitochondrion,Nucleus. |
WB
Western blot analysis of SIRT5 on different lysates with Rabbit anti-SIRT5 antibody at 1/2,000 dilution. Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SIRT5 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
