WB
Western blot analysis of SIRT5 using anti-SIRT5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIRT5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SIRT5 using anti-SIRT5 antibody. SIRT5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SIRT5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SIRT5 using anti-SIRT5 antibody. SIRT5 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-SIRT5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of Daudi cells using anti-SIRT5 antibody. Overlay histogram showing Daudi cells stained with Rabbit polyclonal antibody to SIRT5 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRT5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SIRT5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SIRT5 recombinant protein (Position: D81-S310). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT5 |
|---|---|
| Gene Synonyms | SIR2L5 |
| Gene Full Name | sirtuin 5 |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| Alternative Names | SIR2 like protein 5 SIR2L5 SIRT5 |
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Cytoplasm,Mitochondrion,Nucleus |
WB
Western blot analysis of SIRT5 using anti-SIRT5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIRT5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SIRT5 using anti-SIRT5 antibody. SIRT5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-SIRT5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SIRT5 using anti-SIRT5 antibody. SIRT5 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-SIRT5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of Daudi cells using anti-SIRT5 antibody. Overlay histogram showing Daudi cells stained with Rabbit polyclonal antibody to SIRT5 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRT5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
