SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family.1) Frye, R.A. et al., (2000) Res. Commun. 273 (2), 793-798.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SIRT5 antibody can be used for detection of SIRT5 by ELISA at 1:12500. SIRT5 antibody can be used for detection of SIRT5 by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Jurkat 1.0-2.0 ug/ml.
Application
Antibody used in IHC on Human Lung.
Application
Antibody used in IHC on Human kidney.
Application Notes
SIRT5 antibody can be used for detection of SIRT5 by ELISA at 1:12500. SIRT5 antibody can be used for detection of SIRT5 by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt