Application
Antibody used in WB on Human HEK293T at: 1:200 (Lane 1: Untransfected HEK293T lysate, Lane 2: mSIRT5-FLAG transfected HEK293T lysate).Application
Antibody used in WB on Human Heart at 5.0 ug/ml.| Product Name | SIRT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIRT5, SIR2L5 |
| Product description | SIRT5 is included in class III of the sirtuin family which ischaracterized by a sirtuin core domain. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.1) Mahlknecht, U., et al., (2006) Cytogenet. Genome Res. 112 (3-4), 208-212. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SIRT5 antibody can be used for detection of SIRT5 by ELISA at 1:62500. SIRT5 antibody can be used for detection of SIRT5 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIRT5 |
|---|---|
| Alternative Names | SIRT5 SIR2L5 |
| Molecular Weight(MW) | 33 kDa |
Application
Antibody used in WB on Human HEK293T at: 1:200 (Lane 1: Untransfected HEK293T lysate, Lane 2: mSIRT5-FLAG transfected HEK293T lysate).
Application
Antibody used in WB on Human Heart at 5.0 ug/ml.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | SIRT5 antibody can be used for detection of SIRT5 by ELISA at 1:62500. SIRT5 antibody can be used for detection of SIRT5 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SIRT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
