| Product Name | Sirtuin 6 Antibody [S6R82-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards 'Lys-9' and 'Lys-56' of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-alpha. |
| Immunogen | Recombinant human sirtuin 6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SIRT6 |
|---|---|
| Alternative Names | SIRT6 NAD-dependent Deacetylase Sirtuin-6 Mono-ADP-ribosyltransferase Sirtuin 6 SIR2-like Protein 6 SIR2L6 |
| Tissue Specificity | Recognizes human sirtuin 6. Detects a band of ~47kDa by Western blot. |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
