Application
Fluorescent confocal image of Hela cell stained with SIX5 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloidinApplication
SIX5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | SIX5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2.1) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) |
| Immunogen | This SIX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human SIX5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IF,IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIX5 |
|---|---|
| Alternative Names | Homeobox protein SIX5 DM locus-associated homeodomain protein Sine oculis homeobox homolog 5 SIX5 DMAHP |
| Molecular Weight(MW) | 75 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Fluorescent confocal image of Hela cell stained with SIX5 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin
Application
SIX5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
