Application
Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SKA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SKA1 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.1) Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21. |
| Immunogen | SKA1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA1. The immunogen is located within amino acids 180 - 230 of SKA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5 - 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SKA1 |
|---|---|
| Alternative Names | SKA1 Antibody: C18orf24 C18orf24 Spindle and kinetochore-associated protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (85%) |
Application
Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5 - 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SKA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SKA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
