Application
Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SKA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SKA2 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.1) Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21. |
| Immunogen | SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2. The immunogen is located within the first 50 amino acids of SKA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SKA2 |
|---|---|
| Alternative Names | SKA2 Antibody: FAM33A FAM33A Spindle and kinetochore-associated protein 2 Protein FAM33A |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (93%), Rat: (80%) |
Application
Western blot analysis of SKA2 in 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | SKA2 antibody can be used for detection of SKA2 by Western blot at 0.5 - 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SKA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SKA2 Antibody is supplied in PBS containing 0.02% sodium azide. |
