Application
Western blot analysis of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | SKA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SKA3 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.1) Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21. |
| Immunogen | SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3. The immunogen is located within amino acids 290 - 340 of SKA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SKA3 |
|---|---|
| Alternative Names | SKA3 Antibody: RAMA1 C13orf3 RAMA1 Spindle and kinetochore-associated protein 3 |
Application
Western blot analysis of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SKA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SKA3 Antibody is supplied in PBS containing 0.02% sodium azide. |
