Application
Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μg/ml.Application
Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μg/ml.Application
Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μg/ml.| Product Name | SKI2W Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1,2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3,4).1) Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6. |
| Immunogen | SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W. The immunogen is located within amino acids 60 - 110 of SKI2W. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SKIV2L |
|---|---|
| Alternative Names | Superkiller viralicidic activity 2-like DDX13 Helicase-like protein Helicase SKI2W HLP SKI2 SKIV2 SKIV2L |
| Molecular Weight(MW) | Predicted: 113, 137 kDa Observed: 135 kDa |
| Sequence Similarities | 2) Cat. No. 10-801 - Human Stomach Tissue Slide |
| Tissue Specificity | SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μg/ml.
Application
Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μg/ml.
Application
Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1415 - Mouse Stomach Tissue Lysate |
|---|---|
| Application Notes | SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | SKI2W antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SKI2W antibody is supplied in PBS containing 0.02% sodium azide. |
