Application
Western blot analysis of anti-SKP1A Pab in CEM cell line lysate (35ug/lane).| Product Name | SKP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, p19A, Organ of Corti protein 2, OCP-2, Organ of Corti protein II, OCP-II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-lik |
| Product description | SKP1A is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin proteolysis through an F-box motif. SKP1A also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor.1) Wu, G., et al., Mol. Cell 11(6):1445-1456 (2003). |
| Immunogen | This SKP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the Central region of human SKP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SKP1 |
|---|---|
| Alternative Names | S-phase kinase-associated protein 1 Cyclin-A/CDK2-associated protein p19 p19A Organ of Corti protein 2 OCP-2 Organ of Corti protein II OCP-II RNA polymerase II elongation factor-like protein SIII Transcription elongation factor B polypeptide 1-lik |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of anti-SKP1A Pab in CEM cell line lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
