Application
Immunocytochemistry of SLAMF3 in 293 cells with SLAMF3 antibody at 10 μg/mL.Application
Immunofluorescence of SLAMF3 in 293 cells with SLAMF3 antibody at 20 μg/mL.Application
Western blot analysis of SLAMF3 in 293 cell lysate with SLAMF3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | SLAMF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLAMF3 Antibody: hly9, mLY9, CD229, SLAMF3, T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9 |
| Product description | SLAMF3 Antibody: The signaling lymphocyte-activation molecule family member 3 (SLAMF3), also known as LY9, is a cell surface receptor that is expressed on T and B lymphocytes and belongs to the CD150/SLAMF1 receptor family. SLAMF3 was identified through a yeast two-hybrid screening in which SLAMF3 bound to the X-linked lymphoproliferative disease gene product SAP, suggesting that in activated T cells, SLAMF3 signaling can be triggered SAP. Its expression on the cell surface appears to be regulated via its interaction with the clathrin-associated adaptor complex 2 (AP-2). Despite its similarity to SLAMF1 in structure and interactions with SAP, SLAMF3-deficient mice do not exhibit phenotypic characteristics associated with SLAMF1- and SAP-deficient mice, suggesting that SLAMF3 plays other roles in T cell activation.1) Sayos J, Martin M, Chen A, et al. Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP. Blood 2001; 97:3867-74. |
| Immunogen | SLAMF3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SLAMF3. The immunogen is located within amino acids 520 - 570 of SLAMF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| SLAMF3 antibody can be used for detection of SLAMF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LY9 |
|---|---|
| Alternative Names | SLAMF3 Antibody: hly9 mLY9 CD229 SLAMF3 T-lymphocyte surface antigen Ly-9 Cell surface molecule Ly-9 |
| Sequence Similarities | 2) Cat. No. 17-010 - HEK293 Cell Slide |
| Tissue Specificity | At least three isoforms of SLAMF3 are known to exist. SLAMF3 antibody is predicted to not cross-react with other SLAM protein family members. |
Application
Immunocytochemistry of SLAMF3 in 293 cells with SLAMF3 antibody at 10 μg/mL.
Application
Immunofluorescence of SLAMF3 in 293 cells with SLAMF3 antibody at 20 μg/mL.
Application
Western blot analysis of SLAMF3 in 293 cell lysate with SLAMF3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | SLAMF3 antibody can be used for detection of SLAMF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SLAMF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SLAMF3 Antibody is supplied in PBS containing 0.02% sodium azide. |
