Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | SLC12A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SLC12A1, BSC1, MGC48843, NKCC2 |
| Product description | The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SLC12A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SLC12A1 antibody can be used for detection of SLC12A1 by ELISA at 1:312500. SLC12A1 antibody can be used for detection of SLC12A1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC12A1 |
|---|---|
| Alternative Names | SLC12A1 BSC1 MGC48843 NKCC2 |
| Molecular Weight(MW) | 47 kDa, 121 kDa |
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SLC12A1 antibody can be used for detection of SLC12A1 by ELISA at 1:312500. SLC12A1 antibody can be used for detection of SLC12A1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SLC12A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
